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KMID : 0357419940240010087
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Korean journal of Virology
1994 Volume.24 No. 1 p.87 ~ p.92
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Detection of Hepatitis C Virus RNA in Clinical Sera By Reverse Transcription-Polymerase Chain Reaction
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최철석
김은아/박영석/이경옥/이규범/이연태
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Abstract
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In 152 sera obtained from probable patients with hepatitis seroprevalence of antibody against hepatitis C virus(HCV) by ELISA and the viral genome by reverse transcriptase polymerase chain reaction (RT-PCR) were detected. Anti-HCV positive rate
by
enzyme-linked immunosorbent assay(ELISA) were 23.0%(35/152), hepatitis C virus RNA positive were 40.7%(62/152). Of 152, 25(16.4%) were positive either anti-HCV or RT-PCR. Ten cases(6.5%) were anti HCV positive wherease RT-PCR was shown negative.
While,
37 cases(24.3%) were negative for anti-HCV with positive result for RT-PCR. Eighty sera of 152 were negative by both methods. In this study the PCR method provided higher sensitivity than ELISA method for detection of HCV. It is our concluded
that
the
presence of hepatitis C viral genome detected by RT-PCR in sera is a better test of infectivity than the anti-HCV method.
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